Chemical, Microstructural and Morphological Characterisation of Dentine Caries Simulation by pH-Cycling

In vitro simulation of natural caries is great importance in dental research for the development more effective clinical treatments. The pH-cycling (pHc) procedure consists a dynamic process with alternating de-remineralisation periods. current aims to evaluate effects pHc on mineral dentine properties comparison sound and residual caries. For this purpose, slices from human third molars were submitted cycling periods 14 28 days. chemical composition, morphological microstructural samples examined by infrared Raman spectroscopies, X-ray diffraction, scanning electron microscopy techniques. addition, depth demineralisation front was evaluated Masson’s trichrome (MT) staining. results showed that led notable changes composition crystalline characteristics respect some extent MT revealed yields deeper lesion than 14, simulating potential progression study provide better understanding mechanisms could occur an vivo environment standardised substrate similar